LOCUS       pSC/Ble\1-29-07         9457 bp    DNA     circular     29-JAN-2007
DEFINITION  PSC-BLE.
SOURCE      
  ORGANISM  
COMMENT     
            http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|436613173|
COMMENT     VNTDBDATE|436620018|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSC/Ble 1-29-07|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|L. David Sibley|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTUDF|Organism|4|E.coli|
COMMENT     VNTUDF|Date glycerol made:|1|Unknown|
COMMENT     VNTUDF|Cloned by:|1| Dan Howe|
COMMENT     VNTUDF|Host strain:|1|XL1-Blue|
COMMENT     VNTUDF|Freezer Box:|1|WU#2  4G|
COMMENT     VNTUDF|Vector|1|Stratagene's SuperCos|
COMMENT     VNTUDF|Insert Source|1|SAG1/ble cassette from S1/ble plasmid|
COMMENT     VNTUDF|Insert Size|1|1.5 Kb|
COMMENT     VNTUDF|Insert Coding Function|1|Tn5 ble gene controlled by Toxo SAG1 flanks|
COMMENT     VNTUDF|Usage|1|Vector for constructing cosmid libraries and transfection of Toxoplasma|
COMMENT     VNTUDF|Drug Selection|1|AMP|
COMMENT     VNTUDF|Insert Site|1|destroyed|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 3376 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 1174 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 1 4569 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 93 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #9=(CFSignal (CObList) "COS site 1 (300-311)" 86 0 0 300 311 0 
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COMMENT        #10=(CFSignal (CObList) "COS site 2 (1382-1393)" 18 0 0 1382 1393 0 
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COMMENT        #11=(CFSignal (CObList) "AMP" 86 0 1 8395 9251 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "pUC ori" 40 0 0 7566 8243 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: L. David Sibley" 
COMMENT                          1) 
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COMMENT                          "Created: 01/29/07 09:26AM" 1) 
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COMMENT                          "Last Modified: 01/29/07 11:20AM" 1) 
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FEATURES             Location/Qualifiers
     misc_marker     3037..3374
                     /vntifkey="22"
                     /label=SV40\promoter\(3037-3375)
     misc_recomb     300..311
                     /vntifkey="86"
                     /label=COS\site\1\(300-311)
     loci            1382..1393
                     /vntifkey="18"
                     /label=COS\site\2\(1382-1393)
     misc_recomb     complement(8395..9251)
                     /vntifkey="86"
                     /label=AMP
     STS             7566..8243
                     /vntifkey="40"
                     /label=pUC\ori
     misc_structure  5249..6042
                     /vntifkey="88"
                     /label=NEO
     5'UTR           3375..4186
                     /vntifkey="52"
                     /label=SAG1-5'\UTR\(3375-4186)
     old_sequence    4196..4563
                     /vntifkey="89"
                     /label=ble\(4196-4563)
     3'UTR           4572..4880
                     /vntifkey="50"
                     /label=SAG1-3'\UTR\(4572-4880)
     primer_bind     complement(37..56)
                     /vntifkey="28"
                     /label=T7\Promoter\(37-56)
     primer_bind     15..33
                     /vntifkey="28"
                     /label=T3\Promoter\(15-33)
BASE COUNT     2274 a      2457 c      2395 g      2329 t         2 others
ORIGIN
        1 gaattcgcgg ccgcaattaa ccctcactaa agggatccct atagtgagtc gtattatgcg 
       61 gccgcgaatt ctcatgtttg accgcttatc atcgataagc tctgcttttt gttgacttcc 
      121 attgttcatt ccacggacaa aaacagagaa aggaaacgac agaggccaaa aagctcgctt 
      181 tcagcacctg tcgtttcctt tcttttcaga gggtatttta aataaaaaca ttaagttatg 
      241 acgaagaaga acggaaacgc cttaaaccgg aaaattttca taaatagcga aaacccgcga 
      301 ggtcgccgcc ccgtaacaag gcggatcgcc ggaaaggacc cgcaaatgat aataattatc 
      361 aattgcatac tatcgacggc actgctgcca gataacacca ccggggaaac attccatcat 
      421 gatggccgtg cggacatagg aagccagttc atccatcgct ttcttgtctg ctgccatttg 
      481 ctttgtgaca tccagcgccg cacattcagc agcgtttttc agcgcgtttt cgatcaacgt 
      541 ttcaatgttg gtatcaacac caggtttaac tttgaactta tcggcactga cggttacctt 
      601 gttctgcgct ggctcatcac gcaggatacc aaggctgatg ttgtagatat tggtcaccgg 
      661 ctgagggttt tcgattgccg ctgcgtggat agcaccattt gcgatcaggc ngtccttgat 
      721 gaatgacact ccattgcgaa taagttcgaa ggagacggtg tcacgaatgc gctggtccag 
      781 ctcggtcgat tgccttttgt gcagcagagg tatcaatctc aacgccaagg ctcatcgaag 
      841 cgcaatattg ctgctcacca aaacgcgtat tgaccaggtg ttcaacggca aatttctgcc 
      901 cttctgatgt cagaaaggca aagtgatttt ctttctggta ttcagttgct gtgtgtcggt 
      961 ttcagcaaaa ccaagctcgc gcaattcggc tgtgcagatt tagaaggcag atcaccagac 
     1021 agcaacggcc aacggaaaac agcgcataca gaacatccgt cgccgcgccg acaacgtgat 
     1081 aatttttatg acccatgatt tatttccttt tagacgtgag cctgtcgcac agcaaagccg 
     1141 ccgaaagttc ctcgaagcta gcttcagacg tgtctagata cgtctgcttt ttgttgactt 
     1201 ccattgttca ttccacggac aaaaacagag aaaggaaacg acagaggcca aaaagctcgc 
     1261 tttcagcacc tgtcgtttcc tttcttttca gagggtattt taaataaaaa cattaagtta 
     1321 tgacgaagaa gaacggaaac gccttaaacc ggaaaatttt cataaatagc gaaaacccgc 
     1381 gaggtcgccg ccccgtaaca aggcggatcg ccggaaagga cccgcaaatg ataataatta 
     1441 tcaattgcat actatcgacg gcactgctgc cagataacac caccggggaa acattccatc 
     1501 atgatggccg tgcggacata ggaagccagt tcatccatcg ctttcttgtc tgctgccatt 
     1561 tgctttgtga catccagcgc cgcacattca gcagcgtttt tcagcgcgtt ttcgatcaac 
     1621 gtttcaatgt tggtatcaac accaggttta actttgaact tatcggcact gacggttacc 
     1681 ttgttctgcg ctggctcatc acgcaggata ccaaggctga tgttgtagat attggtcacc 
     1741 ggctgagggt tttcgattgc cgctgcgtgg atagcaccat ttgcgatcag gcngtccttg 
     1801 atgaatgaca ctccattgcg aataagttcg aaggagacgg tgtcacgaat gcgctggtcc 
     1861 agctcggtcg attgcctttt gtgcagcaga ggtatcaatc tcaacgccaa ggctcatcga 
     1921 agcgcaatat tgctgctcac caaaacgcgt attgaccagg tgttcaacgg caaatttctg 
     1981 cccttctgat gtcagaaagg caaagtgatt ttctttctgg tattcagttg ctgtgtgtcg 
     2041 gtttcagcaa aaccaagctc gcgcaattcg gctgtgcaga tttagaaggc agatcaccag 
     2101 acagcaacgg ccaacggaaa acagcgcata cagaacatcc gtcgccgcgc cgacaacgtg 
     2161 ataattttta tgacccatga tttatttcct tttagacgtg agcctgtcgc acagcaaagc 
     2221 cgccgaaagt tcctcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt 
     2281 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg 
     2341 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg 
     2401 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct 
     2461 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg 
     2521 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc 
     2581 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt 
     2641 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca 
     2701 gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca 
     2761 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt 
     2821 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc 
     2881 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc 
     2941 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg 
     3001 gcgcatctcg ggcagcgttg ggtcctgcag atccggctgt ggaatgtgtg tcagttaggg 
     3061 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 
     3121 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 
     3181 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 
     3241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 
     3301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 
     3361 ctaggctttt gcaaaagctt ttacatccgt tgccttttcc acggtccgtg atttcatgtg 
     3421 cgtgcagctt caaagactgg tcgttgcgac taataagact gcagtgacag gtcgaatggt 
     3481 gggcaccttg ctgatgacta tctactgcaa agtctgagac aacgaacgaa acttcccaca 
     3541 cgaggcattt gaaactgacg gtgtctaggt aatatgcact gcaagacacg gtactggggc 
     3601 ctcgctgaat taggggccga tctcgttgcc ctatcagtgc tcacagtgcc gcaacgtaac 
     3661 accagggcag gttcttgaca gtggcaacaa tgtgcgacgg gcgtgtgaac gtttcgtagt 
     3721 catagcgcta gcacgtacct agccacatgg tcgtgaggag ctttaccatg cgtctagcta 
     3781 gaaggtggat gcgggacacg ccttcctggc ctttggctcc cgagacgcgt gttctaacca 
     3841 caaaccttga gacgcgtgtt ccaaccacgc accctgacac gcgtgttcca accacgcacc 
     3901 ctgagacgcg tgttctaacc acgcaccctg agacgcgtgt tctaaccacg caccctgaga 
     3961 cgcgtgttct gccgcacaat gtgcacctgt aggaagctgt agtcactgct gattctcact 
     4021 gttctcggca agggccgacg accggagtac agtttttgtg ggcagagccg ttgtgcagct 
     4081 ttccgttctt ctcggttgtg tcacatgtgt cattgtcgtg taaacacacg gttgtatgtc 
     4141 ggtttcgctg caccacttca ttatttcttc tggttttttg acgagtatgc atgaccaagc 
     4201 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 
     4261 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 
     4321 ggagttcttc gcccaccccg ggctcgatcc cctcgcgagt tggttcagct gctgcctgag 
     4381 gctggacgac ctcgcggagt tctaccggca gtgcaaatcc gtcggcatcc aggaaaccag 
     4441 cagcggctat ccgcgcatcc atgcccccga actgcaggag tggggaggca cgatggccgc 
     4501 tttggtcgac ccggacggga cgctcctgcg cctgatacag aacgaattgc ttgcaggcat 
     4561 ctcttaatta atcaccgttg tgctcacttc tcaaatcgac aaaggaaaca cacttcgtgc 
     4621 agcatgtgcc ccattataaa gaaactgagt tgttccgttg tggcttgcag gtgtcacatc 
     4681 cacaaaaacc ggccgactct aaataggagt gtttcgcagc aagcagcgaa agtttatgac 
     4741 tgggtccgaa tctctgaacg gatgtgtggc ggacctggct gatgttgatc gccgtcgaca 
     4801 cacgcgccac atgggtcaat acacaagaca gctatcagtt gttttagtcg aaccggttaa 
     4861 cacaattctt gcccccccga gggggatcgg tggagcttca cgctgccgca agcactcagg 
     4921 gcgcaagggc tgctaaagga agcggaacac gtagaaagcc agtccgcaga aacggtgctg 
     4981 accccggatg aatgtcagct actgggctat ctggacaagg gaaaacgcaa gcgcaaagag 
     5041 aaagcaggta gcttgcagtg ggcttacatg gcgatagcta gactgggcgg ttttatggac 
     5101 agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga agccctgcaa 
     5161 agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat caagatctga 
     5221 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 
     5281 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 
     5341 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 
     5401 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 
     5461 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 
     5521 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 
     5581 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 
     5641 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 
     5701 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 
     5761 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 
     5821 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 
     5881 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 
     5941 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 
     6001 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 
     6061 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 
     6121 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 
     6181 cgcggggatc tcatgctgga gttcttcgcc caccccgggc tcgatcccct cgcgagttgg 
     6241 ttcagctgct gcctgaggct ggacgacctc gcggagttct accggcagtg caaatccgtc 
     6301 ggcatccagg aaaccagcag cggctatccg cgcatccatg cccccgaact gcaggagtgg 
     6361 ggaggcacga tggccgcttt ggtccggatc tttgtgaagg aaccttactt ctgtggtgtg 
     6421 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta 
     6481 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat 
     6541 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca 
     6601 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca 
     6661 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg 
     6721 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa 
     6781 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg 
     6841 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct 
     6901 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt 
     6961 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt 
     7021 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa 
     7081 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag 
     7141 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     7201 gtccaaactc atcaatgtat cttatcatgt ctggatctga cgggtgcgca tgatcgtgct 
     7261 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc 
     7321 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac 
     7381 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgct 
     7441 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     7501 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     7561 acatgtgagc aaaaggccag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 
     7621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 
     7681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 
     7741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 
     7801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 
     7861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 
     7921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 
     7981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 
     8041 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 
     8101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 
     8161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 
     8221 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 
     8281 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 
     8341 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 
     8401 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 
     8461 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 
     8521 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 
     8581 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 
     8641 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 
     8701 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 
     8761 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 
     8821 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 
     8881 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 
     8941 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 
     9001 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
     9061 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
     9121 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
     9181 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
     9241 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
     9301 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
     9361 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 
     9421 taaaaatagg cgtatcacga ggccctttcg tcttcaa 
//

